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ProductsBack/Goat anti-Neurexin 1

Goat anti-Neurexin 1

EB07831
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetNRXN1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Neurexin 1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID9378
  • Target name
    NRXN1
  • Target description
    neurexin 1
  • Target synonyms
    Hs.22998; neurexin I; neurexin-1; PTHSL2; SCZD17
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004792.1 ; NP_620072.1. GeneIDs all Nonhuman: 18189 (mouse); 60391 (rat);. Purification: Antigen affinity purified. Names and symbols: neurexin 1; DKFZp313P2036; NRXN1; FLJ35941; Hs.22998; KIAA0578; neurexin I
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Neurexin 1/NRXN1 using anti-Neurexin 1/NRXN1 antibody (A01490-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurexin 1/NRXN1 antigen affinity purified polyclonal antibody (Catalog # A01490-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurexin 1/NRXN1 at approximately 160-170 kDa. The expected band size for Neurexin 1/NRXN1 is at 50 kDa.
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