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ProductsBack/NRXN1 / Neurexin 1 Antibody

NRXN1 / Neurexin 1 Antibody

LS-C496777
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNRXN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NRXN1 / Neurexin 1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 15kDa/46kDa/161kDa/164kDa/169kDa, while the observed MW by Western blot was 140kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 15kDa/46kDa/161kDa/164kDa/169kDa, while the observed MW by Western blot was 140kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9378
  • Target name
    NRXN1
  • Target description
    neurexin 1
  • Target synonyms
    Hs.22998; neurexin I; neurexin-1; PTHSL2; SCZD17
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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WB analysis of HEK293 overexpressing human Neurexin 1 with DYKDDDDK tag using GTX89028 Neurexin 1 antibody, C-term. Lane A : primary antibody Lane B : Mock-transfected HEK293 probed with primary antibody Lane C : Tag antibody Dilution : 0.5microg/ml Loading : 10microg protein in RIPA buffer
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Figure 1. Western blot analysis of Neurexin 1/NRXN1 using anti-Neurexin 1/NRXN1 antibody (A01490-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurexin 1/NRXN1 antigen affinity purified polyclonal antibody (Catalog # A01490-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurexin 1/NRXN1 at approximately 160-170 kDa. The expected band size for Neurexin 1/NRXN1 is at 50 kDa.
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