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Goat anti-PAX3

EB06343
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Rat
TargetPAX3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PAX3
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5077
  • Target name
    PAX3
  • Target description
    paired box 3
  • Target synonyms
    CDHS, HUP2, PAX-3, WS1, WS3, paired box protein Pax-3, paired box homeotic gene 3, paired domain gene 3, paired domain gene HuP2, transcriptional factor PAX3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1. GeneIDs all Nonhuman: 18505 (mouse); 114502 (rat);. Purification: Antigen affinity purified. Names and symbols: PAX3; WS1; CDHS; HUP2; paired box gene 3 (Waardenburg syndrome 1); paired domain gene 3; paired box homeotic gene 3 (Waardenburg syndrome 1); paired box 3; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box homeotic gene 3; paired domain ge
  • Reactivity
    Bovine, Human, Rat
  • Reactivity Supplier
    Human, Rat, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Western Blot Positive WB detected in: A2780 whole cell lysate, A375 whole cell lysate All lanes: PAX3 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 25, 23, 45, 46, 54, 56 kDa Observed band size: 53 kDa
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Figure 1. Western blot analysis of PAX3 using anti-PAX3 antibody (A00285-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX3 antigen affinity purified polyclonal antibody (Catalog # A00285-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX3 at approximately 53KD. The expected band size for PAX3 is at 53KD.
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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX3 antibody [C1C3] (GTX114094) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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