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Goat anti-PDE5A

EB06645
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPDE5A
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PDE5A Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB-Trf, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID8654
  • Target name
    PDE5A
  • Target description
    phosphodiesterase 5A
  • Target synonyms
    CGB-PDE; cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase; cGMP-specific 3',5'-cyclic phosphodiesterase; cGMP-specific phosphodiesterase PDE5A2; cGMP-specific phosphodiesterase type 5A; CN5A; PDE5; phosphodiesterase 5A, cGMP-specific; phosphodiesterase isozyme 5
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001074.2; NP_246273.2; NP_236914.2; (NP_237223). Purification: Antigen affinity purified. Names and symbols: PDE5A; CN5A; PDE5; PDE5A1; CGB-PDE; phosphodiesterase 5A, cGMP-specific; cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
  • Reactivity Supplier
    Human, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Human HT1080 cell lysates; Lane 2: Human SH-SY5Y cell lysates probed with PDE5A Polyclonal Antibody, Unconjugated (bs-2349R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 2. IHC analysis of PDE5A using anti-PDE5A antibody (A02490-2). PDE5A was detected in paraffin-embedded section of human thyroid cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-PDE5A Antibody (A02490-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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