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ProductsBack/Goat anti-PDGFB precursor

Goat anti-PDGFB precursor

EB09553
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
Product group Antibodies
TargetPDGFB
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PDGFB precursor Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the vesicles of MCF7 cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA
  • Applications Supplier
    Pep-ELISA, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5155
  • Target name
    PDGFB
  • Target description
    platelet derived growth factor subunit B
  • Target synonyms
    IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis, platelet-derived growth factor subunit B, PDGF subunit B, PDGF, B chain, becaplermin, epididymis secretory sperm binding protein, platelet-derived growth factor 2, platelet-derived growth factor B chain, platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog), platelet-derived growth factor, beta polypeptide (oncogene SIS), proto-oncogene c-Sis
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002599.1; NP_148937.1. GeneIDs all Nonhuman: 18591 (mouse); 24628 (rat);. Purification: Antigen affinity purified. Names and symbols: PDGFB; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); FLJ12858; PDGF2; SIS; SSV; c-sis; PDGF, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); becaplermin; oncogene SIS; platelet-d
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDGF beta using anti-PDGF beta antibody (A00348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGF beta antigen affinity purified polyclonal antibody (Catalog # A00348) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGF beta at approximately 27KD. The expected band size for PDGF beta is at 27KD.
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