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ProductsBack/Goat anti-Pecam1 / CD31 (mouse)

Goat anti-Pecam1 / CD31 (mouse)

EB12605
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPecam1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Pecam1 / CD31 (mouse) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID18613
  • Target name
    Pecam1
  • Target description
    platelet/endothelial cell adhesion molecule 1
  • Target synonyms
    C85791; Cd31; Pecam; PECAM-1; platelet endothelial cell adhesion molecule
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_032842.2; NP_001027550.1. GeneIDs all Nonhuman: 18613 (mouse);. Purification: Antigen affinity purified. Names and symbols: Pecam1; platelet/endothelial cell adhesion molecule 1; C85791; Cd31; PECAM-1; Pecam; PECAM-1/CD31; platelet endothelial cell adhesion molecule
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD31 using anti-CD31 antibody (A01513-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody (Catalog # A01513-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31 at approximately 120-130 kDa. The expected band size for CD31 is at 83 kDa.
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