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Goat anti-Pleckstrin

EB06169
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPLEK
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Pleckstrin Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, FC, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5341
  • Target name
    PLEK
  • Target description
    pleckstrin
  • Target synonyms
    P47; platelet 47 kDa protein; pleckstrin
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002655.2. Purification: Antigen affinity purified. Names and symbols: PLEK; pleckstrin; P47; p47; FLJ27168
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Pleckstrin/PLEK using anti-Pleckstrin/PLEK antibody (A06656-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human U937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pleckstrin/PLEK antigen affinity purified polyclonal antibody (Catalog # A06656-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pleckstrin/PLEK at approximately 40 kDa. The expected band size for Pleckstrin/PLEK is at 40 kDa.
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Lane 1: Mouse Bone lysates probed with p47 Polyclonal Antibody, Unconjugated (bs-9902R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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