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ProductsBack/Goat Anti-PLG / Plasmin / Plasminogen Secondary Antibody (Azide-free)

Goat Anti-PLG / Plasmin / Plasminogen Secondary Antibody (Azide-free)

LS-C758243
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPLG
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Goat Anti-PLG / Plasmin / Plasminogen Secondary Antibody (Azide-free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5340
  • Target name
    PLG
  • Target description
    plasminogen
  • Target synonyms
    HAE4, plasminogen, plasmin
  • Host
    Goat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA018188LA01HU diluted at 1:1000 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of PLG using anti-PLG antibody (A00702-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse spleen tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLG antigen affinity purified polyclonal antibody (Catalog # A00702-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLG at approximately 90-100 kDa. The expected band size for PLG is at 90-100 kDa.
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Paraformaldehyde-fixed, paraffin embedded human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Plasminogen Polyclonal Antibody, Unconjugated (bs-1828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
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