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Goat anti-PPID / CyP-40

EB07886
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPPID
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PPID / CyP-40 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5481
  • Target name
    PPID
  • Target description
    peptidylprolyl isomerase D
  • Target synonyms
    CYP-40, CYPD, peptidyl-prolyl cis-trans isomerase D, 40 kDa peptidyl-prolyl cis-trans isomerase D, PPIase D, cyclophilin 40, cyclophilin D, cyclophilin-related protein, rotamase D, testicular tissue protein Li 147
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005029.1. GeneIDs all Nonhuman: 67738 (mouse); 361967 (rat);. Purification: Antigen affinity purified. Names and symbols: CYP-40; MGC33096; peptidylprolyl isomerase D (cyclophilin D) ; 40 kDa peptidyl-prolyl cis-trans isomerase D; PPIase; cyclophilin 40; cyclophilin D; cyclophilin-related protein; peptidylprolyl isomerase D; rotamase
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPID using anti-PPID antibody (A02424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPID antigen affinity purified polyclonal antibody (Catalog # A02424) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPID at approximately 38 kDa. The expected band size for PPID is at 41 kDa.
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