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Western Blot analysis of HeLa cells using Cyclophilin D Polyclonal Antibody
Western Blot analysis of HeLa cells using Cyclophilin D Polyclonal Antibody
Western Blot analysis of HeLa cells using Cyclophilin D Polyclonal Antibody

PPID Antibody

CSB-PA004907
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPID
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPID Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5481
  • Target name
    PPID
  • Target description
    peptidylprolyl isomerase D
  • Target synonyms
    40 kDa peptidyl-prolyl cis-trans isomerase D; cyclophilin 40; cyclophilin D; cyclophilin-related protein; CYP-40; CYPD; peptidyl-prolyl cis-trans isomerase D; PPIase D; rotamase D; testicular tissue protein Li 147
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08752
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase D
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPID using anti-PPID antibody (A02424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPID antigen affinity purified polyclonal antibody (Catalog # A02424) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPID at approximately 38 kDa. The expected band size for PPID is at 41 kDa.
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