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Goat anti-Prion Protein (143-153)

EB07297
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPRNP
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Prion Protein (143-153) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5621
  • Target name
    PRNP
  • Target description
    prion protein (Kanno blood group)
  • Target synonyms
    ASCR, AltPrP, CD230, CJD, GSS, KURU, PRIP, PrP, PrP27-30, PrP33-35C, PrPc, p27-30, major prion protein, alternative prion protein, CD230 antigen, prion-related protein
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000302.1. GeneIDs all Nonhuman: 19122 (mouse); 24686 (rat);. Purification: Antigen affinity purified. Names and symbols: PRNP ; prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia) ; ASCR; CD230; CJD; GSS; MGC26679; PRIP; PrP; PrP27-30; PrP33-35C; PrPc ; CD230 antigen; major prion protein; prion protein; prion p
  • Reactivity Supplier
    Human, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRNP using anti-PRNP antibody (PB9783). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRNP antigen affinity purified polyclonal antibody (Catalog # PB9783) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRNP at approximately 30 kDa. The expected band size for PRNP is at 30 kDa.
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