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ProductsBack/Goat anti-PSD3 (aa 327-339) Antibody

Goat anti-PSD3 (aa 327-339) Antibody

EB08278
Everest Biotech
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetPSD3

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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PSD3 (aa 327-339) Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID23362
  • Target name
    PSD3
  • Target description
    pleckstrin and Sec7 domain containing 3
  • Target synonyms
    EFA6D, EFA6R, HCA67, PH and SEC7 domain-containing protein 3, ADP-ribosylation factor guanine nucleotide factor 6, epididymis tissue protein Li 20mP, exchange factor for ADP-ribosylation factor guanine nucleotide factor 6 D, exchange factor for ARF6 D, hepatocellular carcinoma-associated antigen 67, pleckstrin homology and SEC7 domain-containing protein 3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_056125.3; NP_996792.1. Purification: Antigen affinity purified. Names and symbols: PSD3; pleckstrin and Sec7 domain containing 3; DKFZp761K1423; EFA6R; HCA67; ADP-ribosylation factor guanine nucleotide factor 6; hepatocellular carcinoma-associated antigen 67
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSD3 using anti-PSD3 antibody (A10329-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD3 antigen affinity purified polyclonal antibody (Catalog # A10329-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD3 at approximately 130 kDa. The expected band size for PSD3 is at 116 kDa.
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