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ProductsBack/Goat anti-PSME1 (isoform 1)

Goat anti-PSME1 (isoform 1)

EB07761
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPSME1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PSME1 (isoform 1) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5720
  • Target name
    PSME1
  • Target description
    proteasome activator subunit 1
  • Target synonyms
    HEL-S-129m, IFI5111, PA28A, PA28alpha, REGalpha, proteasome activator complex subunit 1, 11S regulator complex subunit alpha, 29-kD MCP activator subunit, IGUP I-5111, activator of multicatalytic protease subunit 1, epididymis secretory sperm binding protein Li 129m, interferon gamma up-regulated I-5111 protein, interferon-gamma IEF SSP 5111, interferon-gamma-inducible protein 5111, proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006254.1. GeneIDs all Nonhuman: 19186 (mouse); 29630 (rat);. Purification: Antigen affinity purified. Names and symbols: PSME1; proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); IFI5111; MGC8628; PA28A; PA28alpha; REGalpha ; 11S regulator complex alpha subunit; 29-kD MCP activator subunit; activator of multicatalytic protease subunit 1; interferon gamma up-r
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSME1 using anti-PSME1 antibody (A04638-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSME1 antigen affinity purified polyclonal antibody (Catalog # A04638-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSME1 at approximately 30 kDa. The expected band size for PSME1 is at 29 kDa.
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