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Goat anti-PTPN6 / SHP1 (internal region)

EB07725
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPTPN6
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PTPN6 / SHP1 (internal region) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5777
  • Target name
    PTPN6
  • Target description
    protein tyrosine phosphatase non-receptor type 6
  • Target synonyms
    HCP; HCPH; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; HPTP1C; protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP-1; PTP-1C; SHP1; SHP-1; SHP-1L; SH-PTP1; tyrosine-protein phosphatase non-receptor type 6
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_536858.1; NP_002822.2. GeneIDs all Nonhuman: 15170 (mouse); 116689 (rat);. Purification: Antigen affinity purified. Names and symbols: PTPN6; SHP1; protein tyrosine phosphatase, non-receptor type 6 ; HCP; HCPH; HPTP1C; PTP-1C; SH-PTP1; SHP-1; SHP-1L; 70 kda SHP-1L protein; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; protein-tyrosine phosphatase 1C
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (A00938-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP1/PTPN6 antigen affinity purified polyclonal antibody (Catalog # A00938-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP1/PTPN6 at approximately 68KD. The expected band size for SHP1/PTPN6 is at 68KD.
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