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Goat anti-Scribble / Vartul

EB06251
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSCRIB
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Scribble / Vartul Antibody
  • Delivery Days Customer
    7
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID23513
  • Target name
    SCRIB
  • Target description
    scribble planar cell polarity protein
  • Target synonyms
    CRIB1, SCRB1, SCRIB1, Vartul, oSCRIB, protein scribble homolog, scribbled homolog, scribbled planar cell polarity protein
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCRIBBLE using anti-SCRIBBLE antibody (A01651). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human 22RV1 whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCRIBBLE antigen affinity purified polyclonal antibody (Catalog # A01651) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCRIBBLE at approximately 240KD. The expected band size for SCRIBBLE is at 175KD.
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