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SCRIB antibody

GTX107692
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSCRIB
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Overview

  • Supplier
    GeneTex
  • Product Name
    SCRIB antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.13 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23513
  • Target name
    SCRIB
  • Target description
    scribble planar cell polarity protein
  • Target synonyms
    CRIB1, SCRB1, SCRIB1, Vartul, oSCRIB, protein scribble homolog, scribbled homolog, scribbled planar cell polarity protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14160
  • Protein Name
    Protein scribble homolog
  • Scientific Description
    SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization (summary by Nola et al., 2008 [PubMed 18716323]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ma J, Du WW, Zeng K, et al. An antisense circular RNA circSCRIB enhances cancer progression by suppressing parental gene splicing and translation. Mol Ther. 2021,29(9):2754-2768. doi: 10.1016/j.ymthe.2021.08.002
    Read this paper
  • Youk J, Kim T, Evans KV, et al. Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. Cell Stem Cell. 2020,27(6):905-919.e10. doi: 10.1016/j.stem.2020.10.004
    Read this paper
  • Saeed M, Kapell S, Hertz NT, et al. Defining the proteolytic landscape during enterovirus infection. PLoS Pathog. 2020,16(9):e1008927. doi: 10.1371/journal.ppat.1008927
    Read this paper

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Figure 1. Western blot analysis of SCRIBBLE using anti-SCRIBBLE antibody (A01651). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human 22RV1 whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCRIBBLE antigen affinity purified polyclonal antibody (Catalog # A01651) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCRIBBLE at approximately 240KD. The expected band size for SCRIBBLE is at 175KD.
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