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ProductsBack/Goat anti-Serotonin receptor 2C / HTR2C

Goat anti-Serotonin receptor 2C / HTR2C

EB06786
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetHTR2C
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Serotonin receptor 2C / HTR2C Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3358
  • Target name
    HTR2C
  • Target description
    5-hydroxytryptamine receptor 2C
  • Target synonyms
    5-HT1C; 5-HT2C; 5HTR2C; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled; 5-hydroxytryptamine receptor 1C; 5-hydroxytryptamine receptor 2C; HTR1C; serotonin 5-HT-1C receptor; serotonin 5-HT-2C receptor
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000859.1. Purification: Antigen affinity purified. Names and symbols: HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C ; HGNC:5295; 5-HT2C; HTR1C; RP11-810O3.1; 5-HTR2C; OTTHUMP00000023868; serotonin 5-HT-2C receptor; serotonin receptor 2C
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of 5-HT2C Receptor/HTR2C using anti-5-HT2C Receptor/HTR2C antibody (A01593-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5-HT2C Receptor/HTR2C antigen affinity purified polyclonal antibody (Catalog # A01593-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5-HT2C Receptor/HTR2C at approximately 75 kDa. The expected band size for 5-HT2C Receptor/HTR2C is at 52 kDa.
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