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ProductsBack/Goat anti-SLC2A4 / GLUT4

Goat anti-SLC2A4 / GLUT4

EB10148
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSLC2A4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SLC2A4 / GLUT4 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6517
  • Target name
    SLC2A4
  • Target description
    solute carrier family 2 member 4
  • Target synonyms
    GLUT4, solute carrier family 2, facilitated glucose transporter member 4, GLUT-4, glucose transporter type 4, insulin-responsive, insulin-responsive glucose transporter type 4, solute carrier family 2 (facilitated glucose transporter), member 4
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001033.1. GeneIDs all Nonhuman: 20528 (mouse); 25139 (rat);. Purification: Antigen affinity purified. Names and symbols: SLC2A4; solute carrier family 2 (facilitated glucose transporter), member 4; GLUT4; glucose transporter 4; insulin-responsive glucose transporter type 4
  • Reactivity Supplier
    Human, Mouse, Rat, Cow, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLUT4 using anti-GLUT4 antibody (PB9109). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat cardiac muscle tissue lysate, lane 2: rat skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT4 antigen affinity purified polyclonal antibody (Catalog # PB9109) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT4 at approximately 55KD. The expected band size for GLUT4 is at 55KD.
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