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ProductsBack/Goat anti-SLC7A7 / y+LAT-1 Antibody

Goat anti-SLC7A7 / y+LAT-1 Antibody

EB12061
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSLC7A7
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SLC7A7 / y+LAT-1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID9056
  • Target name
    SLC7A7
  • Target description
    solute carrier family 7 member 7
  • Target synonyms
    LAT3, LPI, MOP-2, Y+LAT1, y+LAT-1, Y+L amino acid transporter 1, lysinuric protein intolerance (dibasicaminoaciduria II), monocyte amino acid permease 2, solute carrier family 7 (amino acid transporter light chain, y+L system), member 7, solute carrier family 7 (cationic amino acid transporter, y+ system), member 7, y(+)L-type amino acid transporter 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001119577.1. Purification: Antigen affinity purified. Names and symbols: SLC7A7; solute carrier family 7 (amino acid transporter light chain, y+L system), member 7; LAT3; LPI; MOP-2; Y+LAT1; y+LAT-1; Y+L amino acid transporter 1; monocyte amino acid permease 2; solute carrier family 7 (cationic amino acid transporter, y+ syste
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC7A7 using anti-SLC7A7 antibody (A03602-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A7 antigen affinity purified polyclonal antibody (Catalog # A03602-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC7A7 at approximately 60 kDa. The expected band size for SLC7A7 is at 56 kDa.
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