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Western Blot Positive WB detected in: Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue All lanes: SLC7A7 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue All lanes: SLC7A7 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue All lanes: SLC7A7 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa

SLC7A7 Antibody

CSB-PA892349LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC7A7
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC7A7 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9056
  • Target name
    SLC7A7
  • Target description
    solute carrier family 7 member 7
  • Target synonyms
    LAT3, LPI, MOP-2, Y+LAT1, y+LAT-1, Y+L amino acid transporter 1, lysinuric protein intolerance (dibasicaminoaciduria II), monocyte amino acid permease 2, solute carrier family 7 (amino acid transporter light chain, y+L system), member 7, solute carrier family 7 (cationic amino acid transporter, y+ system), member 7, y(+)L-type amino acid transporter 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UM01
  • Protein Name
    Y+L amino acid transporter 1
  • Scientific Description
    Involved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Involved in the transport of L-arginine in monocytes.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of SLC7A7 using anti-SLC7A7 antibody (A03602-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A7 antigen affinity purified polyclonal antibody (Catalog # A03602-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC7A7 at approximately 60 kDa. The expected band size for SLC7A7 is at 56 kDa.
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