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ProductsBack/Goat anti-SRD5A1 / 5-alpha reductase 1

Goat anti-SRD5A1 / 5-alpha reductase 1

EB06638
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSRD5A1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SRD5A1 / 5-alpha reductase 1 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6715
  • Target name
    SRD5A1
  • Target description
    steroid 5 alpha-reductase 1
  • Target synonyms
    S5AR 1, 3-oxo-5-alpha-steroid 4-dehydrogenase 1, SR type 1, steroid 5-alpha-reductase type I, steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001038.1. Purification: Antigen affinity purified. Names and symbols: SRD5A1; steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1); 5-alpha reductase; steroid 5-alpha-reductase type I; 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1; steroid-5-alpha-reductase 1
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Kapp FG, Sommer A, Kiefer T, et al. 5-alpha-reductase type I (SRD5A1) is up-regulated in non-small cell lung cancer but does not impact proliferation, cell cycle distribution or apoptosis. Cancer Cell Int. 2012,12(1):1. doi: 10.1186/1475-2867-12-1
    Read this paper

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Figure 1. Western blot analysis of SRD5A1 using anti-SRD5A1 antibody (A03464-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRD5A1 antigen affinity purified polyclonal antibody (Catalog # A03464-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRD5A1 at approximately 29KD. The expected band size for SRD5A1 is at 29KD.
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