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SRD5A1 Antibody

CAB14787
Assay Genie
ReactivityMouse
Product group Antibodies
TargetSRD5A1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    SRD5A1 Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6715
  • Target name
    SRD5A1
  • Target description
    steroid 5 alpha-reductase 1
  • Target synonyms
    S5AR 1, 3-oxo-5-alpha-steroid 4-dehydrogenase 1, SR type 1, steroid 5-alpha-reductase type I, steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
  • Host
    Rabbit
  • Protein IDP18405
  • Protein Name
    3-oxo-5-alpha-steroid 4-dehydrogenase 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SRD5A1 using anti-SRD5A1 antibody (A03464-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRD5A1 antigen affinity purified polyclonal antibody (Catalog # A03464-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRD5A1 at approximately 29KD. The expected band size for SRD5A1 is at 29KD.
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