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Goat anti-STAP2 / BKS

EB05127
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSTAP2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-STAP2 / BKS Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID55620
  • Target name
    STAP2
  • Target description
    signal transducing adaptor family member 2
  • Target synonyms
    BKS, signal-transducing adaptor protein 2, BRK substrate, breast tumor kinase substrate, brk kinase substrate
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_060190.2; NP_001013863.1. Purification: Antigen affinity purified. Names and symbols: STAP2; BKS; STAP-2; FLJ20234; breast tumor kinase substrate; brk kinase substrate; signal-transducing adaptor protein-2; signal transducing adaptor family member 2
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ikeda O, Mizushima A, Sekine Y, et al. Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells. Cancer Sci. 2011,102(4):756-61. doi: 10.1111/j.1349-7006.2010.01842.x
    Read this paper
  • Ikeda O, Sekine Y, Mizushima A, et al. Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells. J Biol Chem. 2010,285(49):38093-103. doi: 10.1074/jbc.M110.162388
    Read this paper
  • Sekine Y, Ikeda O, Tsuji S, et al. Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells. J Immunol. 2009,183(12):7966-74. doi: 10.4049/jimmunol.0902096
    Read this paper
  • Ikeda O, Miyasaka Y, Sekine Y, et al. STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation. Biochem Biophys Res Commun. 2009,384(1):71-5. doi: 10.1016/j.bbrc.2009.04.076
    Read this paper

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Figure 1. Western blot analysis of STAP2 using anti-STAP2 antibody (A08413-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAP2 antigen affinity purified polyclonal antibody (Catalog # A08413-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAP2 at approximately 50-55 kDa. The expected band size for STAP2 is at 45 kDa.
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