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ProductsBack/Goat anti-TGFBR1 (aa50-63)

Goat anti-TGFBR1 (aa50-63)

EB11053
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTGFBR1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-TGFBR1 (aa50-63) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: This antibody is not recommended for IF testing.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7046
  • Target name
    TGFBR1
  • Target description
    transforming growth factor beta receptor 1
  • Target synonyms
    AAT5; activin A receptor type II-like kinase, 53kDa; activin A receptor type II-like protein kinase of 53kD; activin receptor-like kinase 5; ACVRLK4; ALK5; ALK-5; ESS1; LDS1; LDS1A; LDS2A; MSSE; mutant transforming growth factor beta receptor I; serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TBRI; TBR-i; TGF-beta receptor type-1; TGFR-1; transforming growth factor beta receptor I; transforming growth factor-beta receptor type I
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004603.1; NP_001124388.1. GeneIDs all Nonhuman: 21812 (mouse); 29591 (rat);. Purification: Antigen affinity purified. Names and symbols: TGFBR1; transforming growth factor, beta receptor 1; AAT5; ACVRLK4; ALK-5; ALK5; LDS1A; LDS2A; SKR4; TGFR-1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta type I receptor; activin A receptor type II-like kinase, 53kD; activin A receptor typ
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TGF Beta Receptor I/TGFBR1 using anti-TGF Beta Receptor I/TGFBR1 antibody (A00832). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor I/TGFBR1 antigen affinity purified polyclonal antibody (Catalog # A00832) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor I/TGFBR1 at approximately 56 kDa. The expected band size for TGF Beta Receptor I/TGFBR1 is at 56 kDa.
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