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Western Blot analysis of HepG2 cells using TGFbeta RI Polyclonal Antibody
Western Blot analysis of HepG2 cells using TGFbeta RI Polyclonal Antibody
Western Blot analysis of HepG2 cells using TGFbeta RI Polyclonal Antibody

TGFBR1 Antibody

CSB-PA004276
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetTGFBR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TGFBR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7046
  • Target name
    TGFBR1
  • Target description
    transforming growth factor beta receptor 1
  • Target synonyms
    AAT5; activin A receptor type II-like kinase, 53kDa; activin A receptor type II-like protein kinase of 53kD; activin receptor-like kinase 5; ACVRLK4; ALK5; ALK-5; ESS1; LDS1; LDS1A; LDS2A; MSSE; mutant transforming growth factor beta receptor I; serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TBRI; TBR-i; TGF-beta receptor type-1; TGFR-1; transforming growth factor beta receptor I; transforming growth factor-beta receptor type I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36897
  • Protein Name
    TGF-beta receptor type-1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TGF Beta Receptor I/TGFBR1 using anti-TGF Beta Receptor I/TGFBR1 antibody (A00832). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor I/TGFBR1 antigen affinity purified polyclonal antibody (Catalog # A00832) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor I/TGFBR1 at approximately 56 kDa. The expected band size for TGF Beta Receptor I/TGFBR1 is at 56 kDa.
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