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Goat anti-TIAM1

EB06182
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
Product group Antibodies
TargetTIAM1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-TIAM1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA
  • Applications Supplier
    Pep-ELISA, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7074
  • Target name
    TIAM1
  • Target description
    TIAM Rac1 associated GEF 1
  • Target synonyms
    human T-lymphoma invasion and metastasis inducing TIAM1 protein; rho guanine nucleotide exchange factor TIAM1; T cell lymphoma invasion and metastasis 1; TIAM-1; T-lymphoma invasion and metastasis-inducing protein 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003244; NP_001340613.1; NP_001340614.1; NP_001340615.1; NP_001340616.1. GeneIDs all Nonhuman: 21844 (mouse);. Purification: Antigen affinity purified. Names and symbols: TIAM1; T-cell lymphoma invasion and metastasis 1; human T-lymphoma invasion and metastasis inducing TIAM1 protein
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIAM1 using anti-TIAM1 antibody (PB10103). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIAM1 antigen affinity purified polyclonal antibody (Catalog # PB10103) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIAM1 at approximately 178 kDa. The expected band size for TIAM1 is at 178 kDa.
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