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Mouse anti TIAM

MUB1806P
Nordic-MUbio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetTIAM1
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti TIAM
  • Delivery Days Customer
    7
  • Application Supplier Note
    F5S F6.2 is useful for western blot and immunohistochemistry on frozen sections. Optimal antibody dilution should be determined by titration.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    Immunohistochemistry (frozen);Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F5S F6.2
  • Gene ID7074
  • Target name
    TIAM1
  • Target description
    TIAM Rac1 associated GEF 1
  • Target synonyms
    human T-lymphoma invasion and metastasis inducing TIAM1 protein; rho guanine nucleotide exchange factor TIAM1; T cell lymphoma invasion and metastasis 1; TIAM-1; T-lymphoma invasion and metastasis-inducing protein 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ13009
  • Protein Name
    T-lymphoma invasion and metastasis-inducing protein 1
  • Source
    F5S F6.2 is a Mouse monoclonal IgG2b antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with T-cell lymphoma invasion and metastasis-inducing protein 1 (Tiam1).
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIAM1 using anti-TIAM1 antibody (PB10103). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIAM1 antigen affinity purified polyclonal antibody (Catalog # PB10103) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIAM1 at approximately 178 kDa. The expected band size for TIAM1 is at 178 kDa.
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