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ProductsBack/Goat Anti-Type III Collagen-BIOT

Goat Anti-Type III Collagen-BIOT

1330-08
SouthernBiotech
ApplicationsElectron Microscopy, Flow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCOL3A1
Price on request
0.2 mg
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Goat Anti-Type III Collagen-BIOT
  • Delivery Days Customer
    7
  • Applications
    Electron Microscopy, Flow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Gene ID1281
  • Target name
    COL3A1
  • Target description
    collagen type III alpha 1 chain
  • Target synonyms
    alpha1 (III) collagen; alpha-1 type III collagen; collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; EDS4A; EDSVASC; Ehlers-Danlos syndrome type IV, autosomal dominant; PMGEDSV
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP02461
  • Protein Name
    Collagen alpha-1(III) chain
  • Scientific Description
    Pooled antisera from goats hyperimmunized with type III collagen; purified by affinity chromatography on type III collagen covalently linked to agarose; reacts with conformational determinants on type III collagen; cross-adsorbed against Collagen types I, II, IV, V, and VI for minimal reactivity
  • Source
    Pooled antisera from goats hyperimmunized with type III collagen
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III/COL3A1 antigen affinity purified polyclonal antibody (Catalog # A00788-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen III/COL3A1 at approximately 200 kDa. The expected band size for Collagen III/COL3A1 is at 139 kDa.
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