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ProductsBack/Goat anti-UBE2I, Biotinylated

Goat anti-UBE2I, Biotinylated

EB05759-B
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetUBE2I
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-UBE2I, Biotinylated Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Gene ID7329
  • Target name
    UBE2I
  • Target description
    ubiquitin conjugating enzyme E2 I
  • Target synonyms
    C358B7.1; P18; RING-type E3 SUMO transferase UBC9; SUMO-1-protein ligase; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; UBC9; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin conjugating enzyme E2I; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; ubiquitin-protein ligase I
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003336.1. GeneIDs all Nonhuman: 22196 (mouse); 25573 (rat);. Purification: Antigen affinity purified. Names and symbols: UBE2I; ubiquitin-conjugating enzyme E2I; C358B7.1; P18; UBC9; SUMO-1-protein ligase; SUMO-protein ligase; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubi
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UBC9/UBE2I using anti-UBC9/UBE2I antibody (A02295-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBC9/UBE2I antigen affinity purified polyclonal antibody (A02295-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBC9/UBE2I at approximately 18 kDa. The expected band size for UBC9/UBE2I is at 18 kDa.
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