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Western blot All lanes: UBE2I antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 19 kDa Observed band size: 19 kDa
Western blot All lanes: UBE2I antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 19 kDa Observed band size: 19 kDa
Western blot All lanes: UBE2I antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 19 kDa Observed band size: 19 kDa

UBE2I Antibody

CSB-PA00474A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUBE2I
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Overview

  • Supplier
    Cusabio
  • Product Name
    UBE2I Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7329
  • Target name
    UBE2I
  • Target description
    ubiquitin conjugating enzyme E2 I
  • Target synonyms
    C358B7.1, P18, UBC9, SUMO-conjugating enzyme UBC9, RING-type E3 SUMO transferase UBC9, SUMO-1-protein ligase, SUMO-protein ligase, ubiquitin carrier protein 9, ubiquitin carrier protein I, ubiquitin conjugating enzyme 9, ubiquitin conjugating enzyme E2I, ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), ubiquitin-conjugating enzyme UbcE2A, ubiquitin-like protein SUMO-1 conjugating enzyme, ubiquitin-protein ligase E2I, ubiquitin-protein ligase I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63279
  • Protein Name
    SUMO-conjugating enzyme UBC9
  • Scientific Description
    Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UBC9/UBE2I using anti-UBC9/UBE2I antibody (A02295-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBC9/UBE2I antigen affinity purified polyclonal antibody (A02295-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBC9/UBE2I at approximately 18 kDa. The expected band size for UBC9/UBE2I is at 18 kDa.
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