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Goat anti-VDR

EB06803
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetVDR
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-VDR Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7421
  • Target name
    VDR
  • Target description
    vitamin D receptor
  • Target synonyms
    1,25-dihydroxyvitamin D3 receptor; NR1I1; nuclear receptor subfamily 1 group I member 1; PPP1R163; protein phosphatase 1, regulatory subunit 163; vitamin D (1,25- dihydroxyvitamin D3) receptor; vitamin D nuclear receptor variant 1; vitamin D3 receptor; vitamin D3 receptor isoform VDRA
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000367.1; NP_001017535.1; NP_001351014.1;NP_001017536.1. GeneIDs all Nonhuman: 22337 (mouse); 24873 (rat);. Purification: Antigen affinity purified. Names and symbols: VDR; vitamin D (1,25- dihydroxyvitamin D3) receptor; HGNC:12679; NR1I1; vitamin D (1,25-dihydroxyvitamin D3) receptor
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Vitamin D Receptor/VDR using anti-Vitamin D Receptor/VDR antibody (A00210-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitamin D Receptor/VDR antigen affinity purified polyclonal antibody (Catalog # A00210-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitamin D Receptor/VDR at approximately 60 kDa. The expected band size for Vitamin D Receptor/VDR is at 60 kDa.
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L1 rat brain lysates L2 rat kidney lysates probed with Anti Vitamin D Receptor/VDR Polyclonal Antibody, Unconjugated (bs-2987R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 47kD. Observed band size:47kD.\n
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