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ProductsBack/Goat anti-VMAT2 / SLC18A2
EB06558 (2µg/ml) staining of K562 (A) and Jurkat (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06558 (2µg/ml) staining of K562 (A) and Jurkat (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06558 (2µg/ml) staining of K562 (A) and Jurkat (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Goat anti-VMAT2 / SLC18A2

EB06558
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSLC18A2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-VMAT2 / SLC18A2 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6571
  • Target name
    SLC18A2
  • Target description
    solute carrier family 18 member A2
  • Target synonyms
    PKDYS2, SVAT, SVMT, VAT2, VMAT2, synaptic vesicular amine transporter, monoamine neurotransmitter transporter, solute carrier family 18 (vesicular monoamine transporter), member 2, synaptic vesicle amine transporter, brain, synaptic vesicle monoamine transporter, brain, vesicle monoamine transporter type 2, vesicle monoamine/H+ antiporter, vesicular amine transporter 2, vesicular monoamine transporter 2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003045.2. GeneIDs all Nonhuman: 214084 (mouse); 25549 (rat);. Purification: Antigen affinity purified. Names and symbols: SLC18A2; VMAT2; SVAT; SVMT; VAT2; solute carrier family 18 (vesicular monoamine), member 2; MGC120477; MGC120478; MGC26538
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Differences in the expression of catecholamine-synthesizing enzymes between vesicular monoamine transporter 1- and 2-immunoreactive glomus cells in the rat carotid body. Kato K et al., 2020 Apr, Acta Histochem
    Read more
  • Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons. Zhang S et al., 2015 Mar, Nat Neurosci
    Read more

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Figure 1. Western blot analysis of VMAT2/SLC18A2 using anti-VMAT2/SLC18A2 antibody (A02232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VMAT2/SLC18A2 antigen affinity purified polyclonal antibody (Catalog # A02232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VMAT2/SLC18A2 at approximately 75 kDa. The expected band size for VMAT2/SLC18A2 is at 56 kDa.
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