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ProductsBack/Goat anti-VMAT2 / SLC18A2
EB06558 (2µg/ml) staining of K562 (A) and Jurkat (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06558 (2µg/ml) staining of K562 (A) and Jurkat (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06558 (2µg/ml) staining of K562 (A) and Jurkat (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Goat anti-VMAT2 / SLC18A2

EB06558
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSLC18A2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-VMAT2 / SLC18A2 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6571
  • Target name
    SLC18A2
  • Target description
    solute carrier family 18 member A2
  • Target synonyms
    monoamine neurotransmitter transporter; PKDYS2; solute carrier family 18 (vesicular monoamine transporter), member 2; SVAT; SVMT; synaptic vesicle amine transporter, brain; synaptic vesicle monoamine transporter, brain; synaptic vesicular amine transporter; VAT2; vesicle monoamine transporter type 2; vesicle monoamine/H+ antiporter; vesicular amine transporter 2; VMAT2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003045.2. GeneIDs all Nonhuman: 214084 (mouse); 25549 (rat);. Purification: Antigen affinity purified. Names and symbols: SLC18A2; VMAT2; SVAT; SVMT; VAT2; solute carrier family 18 (vesicular monoamine), member 2; MGC120477; MGC120478; MGC26538
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Differences in the expression of catecholamine-synthesizing enzymes between vesicular monoamine transporter 1- and 2-immunoreactive glomus cells in the rat carotid body. Kato K et al., 2020 Apr, Acta Histochem
    Read more
  • Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons. Zhang S et al., 2015 Mar, Nat Neurosci
    Read more

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Figure 1. Western blot analysis of VMAT2/SLC18A2 using anti-VMAT2/SLC18A2 antibody (A02232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VMAT2/SLC18A2 antigen affinity purified polyclonal antibody (Catalog # A02232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VMAT2/SLC18A2 at approximately 75 kDa. The expected band size for VMAT2/SLC18A2 is at 56 kDa.
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