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Western Blot Positive WB detected in: Jurkat whole cell lysate, HEK293 whole cell lysate, Mouse brain tissue, Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue All lanes: SLC18A2 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 23 kDa Observed band size: 56 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate, HEK293 whole cell lysate, Mouse brain tissue, Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue All lanes: SLC18A2 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 23 kDa Observed band size: 56 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate, HEK293 whole cell lysate, Mouse brain tissue, Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue All lanes: SLC18A2 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 23 kDa Observed band size: 56 kDa

SLC18A2 Antibody

CSB-PA021427LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetSLC18A2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC18A2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6571
  • Target name
    SLC18A2
  • Target description
    solute carrier family 18 member A2
  • Target synonyms
    monoamine neurotransmitter transporter; PKDYS2; solute carrier family 18 (vesicular monoamine transporter), member 2; SVAT; SVMT; synaptic vesicle amine transporter, brain; synaptic vesicle monoamine transporter, brain; synaptic vesicular amine transporter; VAT2; vesicle monoamine transporter type 2; vesicle monoamine/H+ antiporter; vesicular amine transporter 2; VMAT2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05940
  • Protein Name
    Synaptic vesicular amine transporter
  • Scientific Description
    Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VMAT2/SLC18A2 using anti-VMAT2/SLC18A2 antibody (A02232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VMAT2/SLC18A2 antigen affinity purified polyclonal antibody (Catalog # A02232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VMAT2/SLC18A2 at approximately 75 kDa. The expected band size for VMAT2/SLC18A2 is at 56 kDa.
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