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Goat anti-ZAP70

EB11394
Everest Biotech
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetZAP70
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ZAP70 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer). Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7535
  • Target name
    ZAP70
  • Target description
    zeta chain of T cell receptor associated protein kinase 70
  • Target synonyms
    70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; IMD48; SRK; STCD; STD; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001070.2. GeneIDs all Nonhuman: 22637 (mouse); 301348 (rat);. Purification: Antigen affinity purified. Names and symbols: ZAP70; zeta-chain (TCR) associated protein kinase 70kDa; FLJ17670; FLJ17679; SRK; STD; TZK; ZAP-70; 70 kDa zeta-associated protein; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; zeta-chain associated protein kinase, 70kD
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • A high-throughput pipeline for validation of antibodies. Sikorski K et al., 2018 Nov, Nat Methods
    Read more

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Figure 1. Western blot analysis of ZAP70 using anti-ZAP70 antibody (PB9968). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZAP70 antigen affinity purified polyclonal antibody (Catalog # PB9968) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZAP70 at approximately 70 kDa. The expected band size for ZAP70 is at 70 kDa.
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