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Immunofluorescence analysis of HepG2 cells using GOLGA1 Polyclonal Antibody at dilution of 1:150. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using GOLGA1 Polyclonal Antibody at dilution of 1:150. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using GOLGA1 Polyclonal Antibody at dilution of 1:150. Blue: DAPI for nuclear staining.

GOLGA1 Polyclonal Antibody

E-AB-65072
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    GOLGA1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein is associated with Sjogrens syndrome.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Golgin 97/GOLGA1 using anti-Golgin 97/GOLGA1 antibody (A13524-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Golgin 97/GOLGA1 antigen affinity purified polyclonal antibody (Catalog # A13524-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Golgin 97/GOLGA1 at approximately 88-97 kDa. The expected band size for Golgin 97/GOLGA1 is at 88 kDa.
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Golgin 97 antibody [C2C3], C-term (GTX114445) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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