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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA600096LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA600096LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA600096LA01HU at dilution of 1:100

GOLGA3 Antibody

CSB-PA600096LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGOLGA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    GOLGA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2802
  • Target name
    GOLGA3
  • Target description
    golgin A3
  • Target synonyms
    GCP170, MEA-2, golgin subfamily A member 3, Golgi membrane associated protein, Golgi peripheral membrane protein, SY2/SY10 protein, golgi autoantigen, golgin subfamily a, 3, golgi complex-associated protein of 170 kDa, golgin-160, golgin-165, male enhanced antigen-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08378
  • Protein Name
    Golgin subfamily A member 3
  • Scientific Description
    Golgi auto-antigen; probably involved in maintaining Golgi structure.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLGA3 antigen affinity purified polyclonal antibody (Catalog # A07522-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLGA3 at approximately 180 kDa. The expected band size for GOLGA3 is at 167 kDa.
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TargetGOLGA3
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Immunohistochemical staining of human colon, prostate, testis and tonsil using Anti-GOLGA3 antibody HPA039809 (A) shows similar protein distribution across tissues to independent antibody HPA040044 (B).
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Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with GOLGA3 antibody [C3], C-term (GTX100288) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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TargetGOLGA3
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