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GOLGA3 Polyclonal Antibody

BS-13480R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGOLGA3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    GOLGA3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2802
  • Target name
    GOLGA3
  • Target description
    golgin A3
  • Target synonyms
    GCP170; golgi autoantigen, golgin subfamily a, 3; golgi complex-associated protein of 170 kDa; Golgi membrane associated protein; Golgi peripheral membrane protein; golgin subfamily A member 3; golgin-160; golgin-165; male enhanced antigen-2; MEA-2; SY2/SY10 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLGA3 antigen affinity purified polyclonal antibody (Catalog # A07522-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLGA3 at approximately 180 kDa. The expected band size for GOLGA3 is at 167 kDa.
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Immunohistochemical staining of human colon, prostate, testis and tonsil using Anti-GOLGA3 antibody HPA039809 (A) shows similar protein distribution across tissues to independent antibody HPA040044 (B).
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