Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX107702/GTX107702_41458_20181025_IHC-P_1_w_23060120_390.webp "GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![GOLPH2 antibody [C3], C-term detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GOLPH2 protein stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:5000. Red: Phalloidin, a F-actin marker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107702/GTX107702_41458_IFA_w_23060120_437.webp "GOLPH2 antibody [C3], C-term detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GOLPH2 protein stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:5000. Red: Phalloidin, a F-actin marker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.")
![GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX107702/GTX107702_41458_20181025_IHC-P_2_w_23060120_691.webp "GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX107702/GTX107702_41458_20181025_IHC-P_3_w_23060120_659.webp "GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. GOLPH2 antibody [C3], C-term (GTX107702) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX107702/GTX107702_39715_IHC_w_23060120_858.webp "GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. GOLPH2 antibody [C3], C-term (GTX107702) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107702/GTX107702_41458_20180330_WB_KO_watremark_w_23060120_357.webp "Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GOLPH2 antibody [C3], C-term
GTX107702
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetGOLM1
Overview
- SupplierGeneTex
- Product NameGOLPH2 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:5000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID51280
- Target nameGOLM1
- Target descriptiongolgi membrane protein 1
- Target synonymsC9orf155, GOLPH2, GP73, HEL46, PSEC0257, bA379P1.3, Golgi membrane protein 1, epididymis luminal protein 46, golgi membrane protein GP73, golgi phosphoprotein 2, golgi protein, 73-kD
- HostRabbit
- IsotypeIgG
- Protein IDQ8NBJ4
- Protein NameGolgi membrane protein 1
- Scientific DescriptionThe Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chua HH, Chang MH, Chen YH, et al. PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis. Cell Mol Gastroenterol Hepatol. 2023,15(1):121-152. doi: 10.1016/j.jcmgh.2022.09.014Read this paper
- Truberg J, Hobohm L, Jochimsen A, et al. Endogenous tagging reveals a mid-Golgi localization of the glycosyltransferase-cleaving intramembrane protease SPPL3. Biochim Biophys Acta Mol Cell Res. 2022,1869(11):119345. doi: 10.1016/j.bbamcr.2022.119345Read this paper
- Inoue J, Ninomiya M, Umetsu T, et al. Small Interfering RNA Screening for the Small GTPase Rab Proteins Identifies Rab5B as a Major Regulator of Hepatitis B Virus Production. J Virol. 2019,93(15). doi: 10.1128/JVI.00621-19Read this paper
- Ding X, Deng G, Liu J, et al. GOLM1 silencing inhibits the proliferation and motility of human glioblastoma cells via the Wnt/β-catenin signaling pathway. Brain Res. 2019,1717:117-126. doi: 10.1016/j.brainres.2019.03.035Read this paper
- Liu KK, Qiu WR, Naveen Raj E, et al. Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway. Autophagy. 2017,13(1):187-200. doi: 10.1080/15548627.2016.1254864Read this paper
Datasheet
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