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Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate All lanes: GOLPH3 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate All lanes: GOLPH3 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate All lanes: GOLPH3 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa

GOLPH3 Antibody

CSB-PA872494LA01HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGOLPH3
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Overview

  • Supplier
    Cusabio
  • Product Name
    GOLPH3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64083
  • Target name
    GOLPH3
  • Target description
    golgi phosphoprotein 3
  • Target synonyms
    coat protein GPP34; coat-protein; golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3; golgi protein; golgi-associated protein; GOPP1; GPP34; MIDAS; mitochondrial DNA absence factor; Vps74
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H4A6
  • Protein Name
    Golgi phosphoprotein 3
  • Scientific Description
    Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GOLPH3 using anti-GOLPH3 antibody (A01333-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLPH3 antigen affinity purified polyclonal antibody (Catalog # A01333-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLPH3 at approximately 36 kDa. The expected band size for GOLPH3 is at 34 kDa.
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