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GOLPH3 Antibody

CAB13121
Assay Genie
TargetGOLPH3
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    GOLPH3 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID64083
  • Target name
    GOLPH3
  • Target description
    golgi phosphoprotein 3
  • Target synonyms
    GOPP1, GPP34, MIDAS, Vps74, Golgi phosphoprotein 3, coat protein GPP34, coat-protein, golgi peripheral membrane protein 1, 34 kDa, golgi protein, golgi-associated protein, mitochondrial DNA absence factor
  • Host
    Rabbit
  • Protein IDQ9H4A6
  • Protein Name
    Golgi phosphoprotein 3
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate All lanes: GOLPH3 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
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Figure 1. Western blot analysis of GOLPH3 using anti-GOLPH3 antibody (A01333-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLPH3 antigen affinity purified polyclonal antibody (Catalog # A01333-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLPH3 at approximately 36 kDa. The expected band size for GOLPH3 is at 34 kDa.
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Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
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Hela cell lysates probed with GOLPH3 Polyclonal Antibody, Unconjugated (bs-13488R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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