GOLPH3 antibody

GeneTex

GOLPH3 antibody

9

WB: 0.5 - 1 microg/mL. IHC-P: 20 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

GOLPH3

golgi phosphoprotein 3

coat protein GPP34; coat-protein; golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3; golgi protein; golgi-associated protein; GOPP1; GPP34; MIDAS; mitochondrial DNA absence factor; Vps74

Rabbit

IgG

Golgi phosphoprotein 3

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

Human, Mouse, Rat

-20°C or -80°C,2°C to 8°C

12352203