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Western blot All lanes: GOPC antibody at 4ug/ml Lane 1: K562 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 50, 36 kDa Observed band size: 51 kDa
Western blot All lanes: GOPC antibody at 4ug/ml Lane 1: K562 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 50, 36 kDa Observed band size: 51 kDa
Western blot All lanes: GOPC antibody at 4ug/ml Lane 1: K562 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 50, 36 kDa Observed band size: 51 kDa

GOPC Antibody

CSB-PA862075ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGOPC
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Overview

  • Supplier
    Cusabio
  • Product Name
    GOPC Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57120
  • Target name
    GOPC
  • Target description
    golgi associated PDZ and coiled-coil motif containing
  • Target synonyms
    CAL; CFTR-associated ligand; dJ94G16.2; dJ94G16.2 PIST; FIG; fused in glioblastoma; golgi-associated PDZ and coiled-coil motif containing protein; Golgi-associated PDZ and coiled-coil motif-containing protein; GOPC1; PDZ protein interacting specifically with TC10; PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10; PIST
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HD26
  • Protein Name
    Golgi-associated PDZ and coiled-coil motif-containing protein
  • Scientific Description
    Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIST/GOPC using anti-PIST/GOPC antibody (A03660-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIST/GOPC antigen affinity purified polyclonal antibody (Catalog # A03660-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIST/GOPC at approximately 55 kDa. The expected band size for PIST/GOPC is at 55 kDa.
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