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ProductsBack/PIST Recombinant Antibody, AbBy Fluor-405 Conjugated

PIST Recombinant Antibody, AbBy Fluor-405 Conjugated

BSM-61917R-BF405
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetGOPC
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Overview

  • Supplier
    Bioss
  • Product Name
    PIST Recombinant Antibody, AbBy Fluor-405 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB(1:300-5000), IF(), FCM(1ug/Test)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID57120
  • Target name
    GOPC
  • Target description
    golgi associated PDZ and coiled-coil motif containing
  • Target synonyms
    CAL, FIG, GOPC1, PIST, dJ94G16.2, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand, PDZ protein interacting specifically with TC10, PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10, dJ94G16.2 PIST, fused in glioblastoma, golgi-associated PDZ and coiled-coil motif containing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HD26
  • Protein Name
    Golgi-associated PDZ and coiled-coil motif-containing protein
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIST/GOPC using anti-PIST/GOPC antibody (A03660-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIST/GOPC antigen affinity purified polyclonal antibody (Catalog # A03660-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIST/GOPC at approximately 55 kDa. The expected band size for PIST/GOPC is at 55 kDa.
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