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GOT2 antibody

10R-1966
Biosynth
Product group Antibodies
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ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    GOT2 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Western blot All lanes: GOT2 antibody at 3microg/ml Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse muscle tissue Lane 4: Mouse spleen tissue Lane 5: MCF-7 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: LO2 whole cell lysate Lane 8: 293T whole cell lysate Lane 9: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 48, 44 kDa Observed band size: 48 kDa
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Figure 1. Western blot analysis of FABP-1/GOT2 using anti-FABP-1/GOT2 antibody (A05998-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human Caco-2 whole cell lysates, Lane 9: human U937 whole cell lysates, Lane 10: rat brain tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat kidney tissue lysates, Lane 13: rat ovary tissue lysates, Lane 14: mouse brain tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse kidney tissue lysates, Lane 17: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP-1/GOT2 antigen affinity purified polyclonal antibody (Catalog # A05998-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP-1/GOT2 at approximately 41KD. The expected band size for FABP-1/GOT2 is at 41KD.
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