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Western blot All lanes: GOT2 antibody at 3microg/ml Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse muscle tissue Lane 4: Mouse spleen tissue Lane 5: MCF-7 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: LO2 whole cell lysate Lane 8: 293T whole cell lysate Lane 9: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 48, 44 kDa Observed band size: 48 kDa
Western blot All lanes: GOT2 antibody at 3microg/ml Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse muscle tissue Lane 4: Mouse spleen tissue Lane 5: MCF-7 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: LO2 whole cell lysate Lane 8: 293T whole cell lysate Lane 9: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 48, 44 kDa Observed band size: 48 kDa
Western blot All lanes: GOT2 antibody at 3microg/ml Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse muscle tissue Lane 4: Mouse spleen tissue Lane 5: MCF-7 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: LO2 whole cell lysate Lane 8: 293T whole cell lysate Lane 9: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 48, 44 kDa Observed band size: 48 kDa

GOT2 Antibody

CSB-PA03065A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetGOT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GOT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2806
  • Target name
    GOT2
  • Target description
    glutamic-oxaloacetic transaminase 2
  • Target synonyms
    DEE82, KAT4, KATIV, KYAT4, mitAAT, aspartate aminotransferase, mitochondrial, FABP-1, FABPpm, aspartate aminotransferase 2, aspartate transaminase 2, fatty acid-binding protein, glutamate oxaloacetate transaminase 2, glutamic-oxaloacetic transaminase 2, mitochondrial, kynurenine aminotransferase 4, kynurenine aminotransferase IV, kynurenine--oxoglutarate transaminase 4, kynurenine--oxoglutarate transaminase IV, mAspAT, plasma membrane-associated fatty acid-binding protein, transaminase A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00505
  • Protein Name
    Aspartate aminotransferase, mitochondrial
  • Scientific Description
    Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of FABP-1/GOT2 using anti-FABP-1/GOT2 antibody (A05998-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human Caco-2 whole cell lysates, Lane 9: human U937 whole cell lysates, Lane 10: rat brain tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat kidney tissue lysates, Lane 13: rat ovary tissue lysates, Lane 14: mouse brain tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse kidney tissue lysates, Lane 17: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP-1/GOT2 antigen affinity purified polyclonal antibody (Catalog # A05998-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP-1/GOT2 at approximately 41KD. The expected band size for FABP-1/GOT2 is at 41KD.
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