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GPBAR1 / TGR5 Antibody (30-110 aa, N-terminal)

LS-C386396
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetGPBAR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GPBAR1 / TGR5 Antibody (30-110 aa, N-terminal)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    ELISA (1:10000), IF (1:200 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID151306
  • Target name
    GPBAR1
  • Target description
    G protein-coupled bile acid receptor 1
  • Target synonyms
    BG37, GPCR19, GPR131, M-BAR, TGR5, G-protein coupled bile acid receptor 1, G-protein coupled bile acid receptor BG37, G-protein coupled receptor GPCR19, membrane bile acid receptor, membrane-type receptor for bile acids
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GPCR TGR5/GPBAR1 using anti-GPCR TGR5/GPBAR1 antibody (A01958-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR TGR5/GPBAR1 antigen affinity purified polyclonal antibody (Catalog # A01958-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR TGR5/GPBAR1 at approximately 46-48KD. The expected band size for GPCR TGR5/GPBAR1 is at 35KD.
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