ICC/IF analysis of COS7 cells transiently transfected with GPI plasmid using GTX84416 GPI antibody [5A11].
![IHC-P analysis of human prostate carcinoma tissue using GTX84416 GPI antibody [5A11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84416/GTX84416_2691_IHC-P_w_23061420_681.webp "IHC-P analysis of human prostate carcinoma tissue using GTX84416 GPI antibody [5A11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of human liver tissue using GTX84416 GPI antibody [5A11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84416/GTX84416_2692_IHC-P_w_23061420_232.webp "IHC-P analysis of human liver tissue using GTX84416 GPI antibody [5A11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![FACS analysis of Jurkat cells using GTX84416 GPI antibody [5A11]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84416/GTX84416_410_FACS_w_23061420_772.webp "FACS analysis of Jurkat cells using GTX84416 GPI antibody [5A11]. Red : Primary antibody Blue : Negative control antibody")
![WB analysis of wild type and GPI knock out 293T cell lysate(10microg per lane) using GTX84416 GPI antibody [5A11]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84416/GTX84416_4353_20181228_WB_w_23061420_847.webp "WB analysis of wild type and GPI knock out 293T cell lysate(10microg per lane) using GTX84416 GPI antibody [5A11]. Dilution : 1:500")
![WB analysis of HEK293T cells transfected with GPI plasmid (Right) or empty vector (Left) for 48 hrs using GTX84416 GPI antibody [5A11]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84416/GTX84416_4354_WB_w_23061420_754.webp "WB analysis of HEK293T cells transfected with GPI plasmid (Right) or empty vector (Left) for 48 hrs using GTX84416 GPI antibody [5A11]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX84416 GPI antibody [5A11]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84416/GTX84416_4355_WB_w_23061420_615.webp "WB analysis of various cell lines using GTX84416 GPI antibody [5A11]. Loading : 35 ug per lane")
ICC/IF analysis of COS7 cells transiently transfected with GPI plasmid using GTX84416 GPI antibody [5A11].
GPI antibody [5A11]
GTX84416
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey
TargetGPI
Overview
- SupplierGeneTex
- Product NameGPI antibody [5A11] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5A11
- Concentration0.72 mg/ml
- ConjugateUnconjugated
- Gene ID2821
- Target nameGPI
- Target descriptionglucose-6-phosphate isomerase
- Target synonymsAMF; autocrine motility factor; glucose-6-phosphate isomerase; GNPI; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; NLK; oxoisomerase; PGI; PHI; phosphoglucose isomerase; phosphohexomutase; phosphohexose isomerase; phosphosaccharomutase; SA36; SA-36; sperm antigen-36
- HostMouse
- IsotypeIgG2b
- Protein IDP06744
- Protein NameGlucose-6-phosphate isomerase
- Scientific DescriptionBesides its role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
- ReactivityCanine, Human, Monkey
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203