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Western blot All lanes: GPR161 antibody at 2microg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 59, 46, 44, 50, 47, 61 kDa Observed band size: 30 kDa
Western blot All lanes: GPR161 antibody at 2microg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 59, 46, 44, 50, 47, 61 kDa Observed band size: 30 kDa
Western blot All lanes: GPR161 antibody at 2microg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 59, 46, 44, 50, 47, 61 kDa Observed band size: 30 kDa

GPR161 Antibody

CSB-PA04114A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGPR161
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Overview

  • Supplier
    Cusabio
  • Product Name
    GPR161 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23432
  • Target name
    GPR161
  • Target description
    G protein-coupled receptor 161
  • Target synonyms
    G-protein coupled receptor 161; G-protein coupled receptor RE2; RE2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N6U8
  • Protein Name
    G-protein coupled receptor 161
  • Scientific Description
    Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling. In presence of SHH, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. Its ligand is unknown (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GPR161 using anti-GPR161 antibody (A10567-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: huamn SiHa whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPR161 antigen affinity purified polyclonal antibody (Catalog # A10567-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPR161 at approximately 60 kDa. The expected band size for GPR161 is at 60 kDa.
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