![GPR30 antibody detects GPR30 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPR30 stained by GPR30 antibody (GTX107748) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). GPR30 antibody detects GPR30 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPR30 stained by GPR30 antibody (GTX107748) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX107748/GTX107748_43160_20181115_ICC_IF_R_w_23060120_543.webp)
GPR30 antibody detects GPR30 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPR30 stained by GPR30 antibody (GTX107748) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GPR30 antibody [C2C3], C-term
GTX107748
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGPER1
Overview
- SupplierGeneTex
- Product NameGPR30 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.26 mg/ml
- ConjugateUnconjugated
- Gene ID2852
- Target nameGPER1
- Target descriptionG protein-coupled estrogen receptor 1
- Target synonymsCEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR, mER, G-protein coupled estrogen receptor 1, G protein-coupled receptor 30, IL8-related receptor DRY12, chemoattractant receptor-like 2, chemokine receptor-like 2, constitutively expressed peptide-like receptor, flow-induced endothelial G-protein coupled receptor 1, heptahelix receptor, lymphocyte-derived G-protein coupled receptor, membrane estrogen receptor
- HostRabbit
- IsotypeIgG
- Protein IDQ99527
- Protein NameG-protein coupled estrogen receptor 1
- Scientific DescriptionThis gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![GPR30 antibody [C2C3], C-term detects GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GPR30 antibody [C2C3], C-term detects GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX107748/GTX107748_44587_20220415_IHC-P_w_23060120_659.webp)


![GPR30 antibody [C2C3], C-term detects GPR30 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GPR30 antibody [C2C3], C-term detects GPR30 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX107748/GTX107748_44629_20231229_IHC-P_multiple_M_24021917_291.webp)




![Non-transfected (–) and transfected (+) bolied and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Non-transfected (–) and transfected (+) bolied and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107748/GTX107748_44755_20240705_WB_B_ub_24070822_140.webp)







