
GPR30 antibody detects GPR30 protein by western blot analysis. A. 50 μg 293T whole cell lysate/extract B. 50 μg whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling) C. 50 μg whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling) 5% SDS-PAGE GPR30 antibody (GTX100001) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GPR30 antibody
GTX100001
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Porcine
TargetGPER1
Overview
- SupplierGeneTex
- Product NameGPR30 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2852
- Target nameGPER1
- Target descriptionG protein-coupled estrogen receptor 1
- Target synonymsCEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR, mER, G-protein coupled estrogen receptor 1, G protein-coupled receptor 30, IL8-related receptor DRY12, chemoattractant receptor-like 2, chemokine receptor-like 2, constitutively expressed peptide-like receptor, flow-induced endothelial G-protein coupled receptor 1, heptahelix receptor, lymphocyte-derived G-protein coupled receptor, membrane estrogen receptor
- HostRabbit
- IsotypeIgG
- Protein IDQ99527
- Protein NameG-protein coupled estrogen receptor 1
- Scientific DescriptionThis gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq]
- ReactivityHuman, Porcine
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Hoang LN, Lee SJ. Casein kinase 1 controls the shuttling of epidermal growth factor receptor and estrogen receptor in endometrial carcinoma induced by breast cancer hormonal therapy: Relevance of GPER1/Src. Cell Signal. 2023,108:110733. doi: 10.1016/j.cellsig.2023.110733Read this paper
- Tran QK, Firkins R, Giles J, et al. Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1. J Biol Chem. 2016,291(20):10805-23. doi: 10.1074/jbc.M115.697334Read this paper






![GPR30 antibody detects GPR30 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPR30 stained by GPR30 antibody (GTX107748) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX107748/GTX107748_43160_20181115_ICC_IF_R_w_23060120_543.webp)

