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GPR30 antibody detects GPR30 protein by western blot analysis. A. 50 μg 293T whole cell lysate/extract B. 50 μg whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling) C. 50 μg whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling) 5% SDS-PAGE GPR30 antibody (GTX100001) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GPR30 antibody detects GPR30 protein by western blot analysis. A. 50 μg 293T whole cell lysate/extract B. 50 μg whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling) C. 50 μg whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling) 5% SDS-PAGE GPR30 antibody (GTX100001) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GPR30 antibody detects GPR30 protein by western blot analysis. A. 50 μg 293T whole cell lysate/extract B. 50 μg whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling) C. 50 μg whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling) 5% SDS-PAGE GPR30 antibody (GTX100001) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GPR30 antibody

GTX100001
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Porcine
TargetGPER1
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Overview

  • Supplier
    GeneTex
  • Product Name
    GPR30 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2852
  • Target name
    GPER1
  • Target description
    G protein-coupled estrogen receptor 1
  • Target synonyms
    CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR, mER, G-protein coupled estrogen receptor 1, G protein-coupled receptor 30, IL8-related receptor DRY12, chemoattractant receptor-like 2, chemokine receptor-like 2, constitutively expressed peptide-like receptor, flow-induced endothelial G-protein coupled receptor 1, heptahelix receptor, lymphocyte-derived G-protein coupled receptor, membrane estrogen receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99527
  • Protein Name
    G-protein coupled estrogen receptor 1
  • Scientific Description
    This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq]
  • Reactivity
    Human, Porcine
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hoang LN, Lee SJ. Casein kinase 1 controls the shuttling of epidermal growth factor receptor and estrogen receptor in endometrial carcinoma induced by breast cancer hormonal therapy: Relevance of GPER1/Src. Cell Signal. 2023,108:110733. doi: 10.1016/j.cellsig.2023.110733
    Read this paper
  • Tran QK, Firkins R, Giles J, et al. Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1. J Biol Chem. 2016,291(20):10805-23. doi: 10.1074/jbc.M115.697334
    Read this paper